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The characteristics of the plassid pKU10 isolated from Pseudomonas putida KU$16 were investigated and its restriction mp was constructed. The pKU10 plu add was a small R p1mW carrying goes for resistance to ampidWa, tetracyclia, and chore phealool, and cared by treadaent with mitomycia C. The molecular size of pKU10 was estimated to be 9.4Kb. Pseudomonas Strains and E- coil cells could be transformed for antibiotic resistance characters specified by pKU10 plasW DNA. By incompatibility test with other plassids, pKU10 is grouped Into IacP-1. EcoRl, Xhol, Seli, BglH, mad Smal cleaved pKU10 once, while Pstl cleaved at two sites, and Hludlll cleaved at six sites. The restrictioa map was constructed by partial and complete digestion of the purified plasal DNA with single, double, or triple restriction enzymes. Thus, pKU10 is expected to be used for a doming vector iaPseudomonas cells.
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